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THE PROCESS OF 10 STEPS OUTLINES THE WHOLE APPROACH TAKEN:

1. To Isolate "In Vitro" Cell Culture Mesenchymal Stem Cells from Human Umbilical Cord.
2. The Risk Assessment for Mesenchymal Stem Cells
3. Clinics / Research offering regenetive medicine

After research in regenetive medicine was performed for one year, a cost/ feasibility study did not require Mesenchymal Stem Cells to be isolated using a Fluorescence-activated cell sorting machine and Cryogenic freezing of cells reduced cell performance function. The approach was reduced in processes, cost and complexity to offer the same result:

4. Evidence from leading cell biologists that Culture Medium from passaging in Cell Culture contained :
5. Cytokines / Exosomes that brought on the same effect as Mesenchymal Stem Cells, which then can be used for:
6. Treating a multiple range of conditions

This is safely performed Xenogenically from:

7. Porcine (Domestic Pig) Bladder Mesenchymal Stem Cells, expanded in cell culture. This resulted in a minimal operational Laboratory. The treatment was supported with:
8. Hyperbaric Oxygen which has the known benefit of inducing new blood vessels. Also a detailed eye test.

The geriatric patient:

9. Ingo Timmann received transdermal cream, oral drinks and eye baths containing the vital Cytokines created from plastic adherent Mesenchymal Stem Cells

Extracellular Vesicles (EV's) can functionally transfer biologicals, changing the recipient cell phenotype in vitro, in vivo and over a distance. We shall in the future research advanced cell biology to implant new teeth using Tissue Engineering and Regenerative Medicine. The Center for Craniofacial Regeneration, Dr. Jeremy Mao has potentially developed a growth factor-infused, three-dimensional scaffold that has the capability of regenerating an anatomically correct tooth. This shall begin the next process:

10. Deciduous Dental Teeth


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